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By F. F. (Editor); Nord

Advances in Enzymology and comparable components of Molecular Biology is a seminal sequence within the box of biochemistry, supplying researchers entry to authoritative studies of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, delivering an unequalled view of the historic improvement of enzymology. The sequence deals researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic strategy, and their program in either the laboratory and undefined. each one quantity within the sequence positive factors contributions by means of major pioneers and investigators within the box from world wide. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide variety of subject matters and lengthy ancient pedigree, Advances in Enzymology and similar parts of Molecular Biology can be utilized not just through scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally via any scientist attracted to the invention of an enzyme, its houses, and its functions.

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Chromatography of fractions 1OC-115 from step I1 on CM-Sephadex (2-50 (procedure C; step 111). 8). 10 . 8) was started at the fraction indicated with an arrow (right-hand inner scale). 1l-ml fractions were collected. The continuous line indicates the absorption at 280 mp. Circles indicate the acid deoxyribonuclease activity (right-hand outer scale). Fractions 37-48 and 49-61 were processed further. From Bernardi, Bernardi, and Chersi (118). 30 QIORGIO BERNARD1 Fig. 16. Rechromatography of acid deoxyribonuclease fractions A and B on CM-Sephadex C-50.

From Bernardi, Bernardi, and Chersi (118). 28 QIORQIO BERNARD1 -5 A E -i c -1OOL IS 0 FRACTION NUMBER Fig. 14. Chromatography of fractions 50-65 from step I on hydroxyapatite (procedure C; step 11). 8). 35. 24-ml fractions were collected. The continuous line indicates the absorption a t 280 m p . Circles (0) indicate the acid deoxyribonuclease activity (right-hand scale). 82; not shown in the figure). Acid ribonucleaae (0; right-hand inner scale) and basic ribonuclease ( A ; left-hand inner scale) are also shown.

81, 599 (1959). 54. Koerner, J. , and Sinsheimer, R. ; J. Ewl. , 888, 1049 (1957). 55. , Eioehim. Bwphys. Acta, 89, 287 (1958). 56. Kurnick, N. , Arch. Eiochem. , 86, 323 (1959). 97. , J. Ewl. , 384, 3003 (1959). ACID DEOXYRIBONUCLEASE 47 58. , J. Biol. , 916,269 (1955). 59. , J . Biol. , 994, 751 (1957). 60. , J. Biol. ,998,49 (1957). 61. , Biochem. Biophys. Res. , 6, 54 (1961). 62. , and Timasheff, S. , Biochem. Biophys. Res. , 6, 58 (1961). 63. , Biochem. , 8S, 32P (1962). 64. , Nature, 906,779 (1965).

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